Everest Biotech

Affinity purified goat polyclonal antibodies

Specialists in anti-peptide and antigen affinity purified goat polyclonal antibodies since 2000. Everest Biotech’s commitment to quality and continuous improvement has been recognised with ISO 9001:2015 accreditation (Certificate number CA14829).

Alongside recombinant monoclonal antibodies, traditional polyclonals continue to play an important scientific role. Their specific high affinity and sensitivity for different epitopes, within the same sample, provides a cost-effective “monoclonal mix” and increased opportunity of target antigen detection. Our team of expert QC scientists, rigorously test every antibody in selected applications, to maximize batch consistency and value for money.

1. Elite Grade Antibodies

   Guaranteed to work as specified on the datasheet, or your money back- part of our 100% Satisfaction Guarantee. Peptide ELISA data showing they recognise the immunizing peptide. Western blot data showing correct size band on physiologically relevant tissue or cell line. IHC/IF data and specific publications where available. Also available in bulk for assay developers. Elite grade plus antibodies have 3+ applications.

2. Aspiring Grade Antibodies

   Brought quickly to the market for targets that have few or no commercial antibodies available and for which there is little or no expression data in the literature. Supported by peptide ELISA data. Low background but wrong size band or no signal in the range of common cell lines and tissues available for testing. Sold at a significantly reduced price but we are not certain that they will recognize the native protein in any application. Reward for sharing data showing the antibody working successfully - submit your data to support@everestbiotech.com to receive a free antibody of your choice. 

3. Immunising Peptides

   Peptides used in production of antibodies avaiable for blocking experiments. Sold as 100µg of Lyophilised peptide.

4. Shi-fix ™ coverslips

   NEW: High quality PLL-coated COVERSLIPS for Immunofluorescence. Do you wish immunofluorescence of suspension cells was as easy as with adherent cells? Now it is! Shi-fix™ (manufactured by Shikhar Biotech) cover-slips allow suspension cells to be layered on them or directly grown as a monolayer. Easy, hassle-free protocol and no spin columns needed for fixing suspension cells to slides. Just add your cells to the coverslips, wait for 30 mins, wash unbound cells with PBS and proceed to immunostaining (or continue the culture to obtain desired cell densities). These cover-slips can also be used for staining suspension cells for microscopy, or for fixing suspension cells for confocal microscopy. What’s more, you can also stimulate the cells while they are attached on the cover-slip, should you need to do so. You can work on non-adherent cells as if they were adherent!